Rapid purification and characterization of angiotensin converting enzyme inhibitory peptides from lizard fish protein hydrolysates with magnetic affinity separation

•Rapid separation was achieved by application of a magnetic field.•ACE was immobilized on magnetic agarose microspheres as magnetic affinity medium.•The magnetic affinity medium was applied to separate ACE inhibitory peptide.•A new ACE inhibitory peptide was identified.•Magnetic affinity technology can advantageously be applied due to its magnetism. In this study, angiotensin converting enzyme (ACE) inhibitory peptides from lizard fish protein hydrolysate with neutral protease were purified through magnetic affinity separation. Magnetic agarose microsphere was prepared by reverse-phase microemulsion method, and its surface was modified with epoxy groups to immobilize ACE as a magnetic affinity medium (MAM-ACE) and then mixed with lizard fish ultrafiltration hydrolysate (