Transformation of the fungal soybean pathogen Cercospora kikuchii with the selectable marker bar

An improved transformation protocol, utilizing selection for resistance to the herbicide bialaphos, has been developed for the plant pathogenic fungus Cercospora kikuchii. Stable, bialaphos-resistant transformants are recovered at frequencies eight times higher than those achieved with the previous...

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Veröffentlicht in:	Applied and Environmental Microbiology 1994-12, Vol.60 (12), p.4592-4595
Hauptverfasser:	Upchurch, R.G. (ARS, USDA, Raleigh, NC.), Meade, M.J, Hightower, R.C, Thomas, R.S, Callahan, T.M
Format:	Artikel
Sprache:	eng
Schlagworte:	ACILTRANSFERASA ACYLTRANSFERASE ANTIBIOTICOS ANTIBIOTIQUE Biological and medical sciences Biotechnology CERCOSPORA KIKUCHII Fundamental and applied biological sciences. Psychology Fungal plant pathogens GENE Generalities. Techniques GENES Genetic engineering Genetic technics HERBICIDAS HERBICIDE MARCADORES GENETICOS MARQUEUR GENETIQUE Methods. Procedures. Technologies Miscellaneous Mycology Phytopathology. Animal pests. Plant and forest protection RESISTANCE AUX PRODUITS CHIMIQUES RESISTENCIA QUIMICA Synthetic digonucleotides and genes. Sequencing TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE
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Beschreibung
Zusammenfassung:	An improved transformation protocol, utilizing selection for resistance to the herbicide bialaphos, has been developed for the plant pathogenic fungus Cercospora kikuchii. Stable, bialaphos-resistant transformants are recovered at frequencies eight times higher than those achieved with the previous system that was based on selection for benomyl resistance. In addition to C. kikuchii, this improved method can also be used to transform other species of Cercospora
ISSN:	0099-2240 1098-5336
DOI:	10.1128/aem.60.12.4592-4595.1994