L-Phenylalanine production by double auxotrophic analogue resistant mutants of Arthrobacter globiformis
A number of tryptophan plus tyrosine double auxotrophic mutants isolated by the NTG treatment of a glutamate producing strain of Arthrobacter globiformis were found to excrete phenylalanine in a mineral salt medium. By controlling the pH of the medium to near neutrality, the active growth period cou...
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Veröffentlicht in: | Acta biotechnologica 1994, Vol.14 (1), p.87-95 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | A number of tryptophan plus tyrosine double auxotrophic mutants isolated by the NTG treatment of a glutamate producing strain of Arthrobacter globiformis were found to excrete phenylalanine in a mineral salt medium. By controlling the pH of the medium to near neutrality, the active growth period could be extended up to 72 h and more phenylalanine was accumulated compared to the unregulated culture where the growth period took up to 48 h. Under optimum culture conditions, the best double auxotroph (TT‐39) produced 3 g phenylalanine/l. Further improvement of phenylalanine production has been achieved by the step‐by‐step isolation of a mutant resistant to the phenylalanine analogues p‐fluorophenylalanine (PFP) and β‐2‐thienylalanine (TA) from the TT‐39 strain. Under optimum culture conditions, the best double auxotrophic analogue resistant mutant TT‐39 PTr‐21 yielded 8.7 g/l phenylalanine. |
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ISSN: | 0138-4988 1521-3846 |
DOI: | 10.1002/abio.370140114 |