Application of enzyme flow microcalorimetry to the study of microkinetic properties of immobilized biocatalyst

Flow microcalorimeter was used for the study of microkinetic properties of Escherichia coli cells enriched with the penicillin G acylase activity immobilized in calcium pectate gel. The experimental kinetic data were obtained by measurement of the thermometric signal in the microcalorimetric column...

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Veröffentlicht in:	Biotechnology techniques 1994-07, Vol.8 (7), p.497-502
Hauptverfasser:	STEFUCA, V, WELWARDOVA, A, GEMEINER, P, JAKUBOVA, A
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Escherichia coli Fundamental and applied biological sciences. Psychology Immobilization of enzymes and other molecules Immobilization techniques Methods. Procedures. Technologies
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	Flow microcalorimeter was used for the study of microkinetic properties of Escherichia coli cells enriched with the penicillin G acylase activity immobilized in calcium pectate gel. The experimental kinetic data were obtained by measurement of the thermometric signal in the microcalorimetric column with immobilized enzyme and described by the introduced mathematical model involving the mass transfer and reaction kinetic phenomena.
ISSN:	0951-208X 1573-6784
DOI:	10.1007/BF00222843