A method for rapid screening of interactions of pharmacologically active compounds with albumin

[Display omitted] •We developed a method for determination of the association constants.•The method was tested for known selected drugs with albumin.•The method was applied for interaction of new tropane derivatives with albumin. We determine the association constants for ligand–protein complex formation using the flow injection method. We carry out the measurements at high flow rates (F=1mLmin−1) of a carrier phase. Therefore, determination of the association constant takes only a few minutes. Injection of 1nM of the ligand (10μL of 1μM concentration of the ligand solution) is sufficient for a single measurement. This method is tested and verified for a number of complexes of selected drugs (cefaclor, etodolac, sulindac) with albumin (BSA). We obtain K=4.45×103M−1 for cefaclor, K=1.00×105M−1 for etodolac and K=1.03×105M−1 for sulindac in agreement with the literature data. We also determine the association constants of 20 newly synthesized 3β- and 3α-aminotropane derivatives with potential antipsychotic activity – ligands of 5-HT1A, 5-HT2A and D2 receptors with the albumin. Results of the studies reported here indicate that potential antipsychotic drugs bind weakly to the transporter protein (BSA) with K≈102–103M−1. Our method allows measuring K in a wide range of values (102–109M−1). This range depends only on the solubility of the ligand and sensitivity of the detector.