Development of a paper-type tyrosinase biosensor for detection of phenolic compounds

A low‐cost, portable, and disposable paper‐type tyrosinase biosensor was developed for determination of phenolic compounds, using a paper‐strip absorption method. Tyrosinase and a chromophore (3‐methyl‐2‐benzothiazolinone hydrazone) were immobilized on paper strips to manufacture the biosensor, whic...

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Veröffentlicht in:	Biotechnology and applied biochemistry 2015-01, Vol.62 (1), p.132-136
Hauptverfasser:	Senyurt, Özge, Eyidoan, Fusun, Yilmaz, Remziye, Oz, M Tufan, Ozalp, V Cengiz, Arica, Yakup, Oktem, Huseyin A
Format:	Artikel
Sprache:	eng
Schlagworte:	4-chlorophenol Agaricales - enzymology Benzothiazoles - chemistry Biochemistry Biosensing Techniques - methods Biosensors Biotechnology catechol Chlorophenol Chromophores cresol Enzymes, Immobilized - chemistry Enzymes, Immobilized - metabolism Hydrazones - chemistry Kinetics l-dopamine Limit of Detection Monitoring Monophenol Monooxygenase - chemistry Monophenol Monooxygenase - metabolism paper strip phenol Phenols Phenols - analysis Phenols - chemistry Portability Stability Tyrosinase Waste water
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Beschreibung
Zusammenfassung:	A low‐cost, portable, and disposable paper‐type tyrosinase biosensor was developed for determination of phenolic compounds, using a paper‐strip absorption method. Tyrosinase and a chromophore (3‐methyl‐2‐benzothiazolinone hydrazone) were immobilized on paper strips to manufacture the biosensor, which was tested on a nontoxic substrate (l‐dopamine). The biosensor was responsive to phenolic compounds such as 4‐chlorophenol, catechol, m‐cresol, and p‐cresol. The sensor showed stability for 70 days. The developed biosensor can be used for remote on‐site qualitative monitoring of phenolic compounds in wastewater samples.
ISSN:	0885-4513 1470-8744
DOI:	10.1002/bab.1246