A rapid and sensitive hemolysis neutralization assay for palytoxin
G. S. Bignami. A rapid and sensitive hemolysis neutralization assay for palytoxin. Toxicon 31, 817–820, 1993.—The hemolytic action of palytoxin was exploited to develop a simple, sensitive assay with specificity based on a palytoxin neutralizing monoclonal antibody. Suspensions of murine erythrocyte...
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| Veröffentlicht in: | Toxicon (Oxford) 1993-06, Vol.31 (6), p.817-820 |
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| container_title | Toxicon (Oxford) |
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| creator | Bignami, Gary S. |
| description | G. S.
Bignami. A rapid and sensitive hemolysis neutralization assay for palytoxin.
Toxicon
31, 817–820, 1993.—The hemolytic action of palytoxin was exploited to develop a simple, sensitive assay with specificity based on a palytoxin neutralizing monoclonal antibody. Suspensions of murine erythrocytes incubated at 37°C in round-bottom microtiter trays formed visible cell pellets which could be lysed by palytoxin. Hemolysis by palytoxin was time- and temperature-dependent, with a 24 hr detection limit of 1 pg/ml. The assay selectively detected palytoxin in a crude extract of
Palythoa tuberculosa. |
| doi_str_mv | 10.1016/0041-0101(93)90389-Z |
| format | Article |
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Bignami. A rapid and sensitive hemolysis neutralization assay for palytoxin.
Toxicon
31, 817–820, 1993.—The hemolytic action of palytoxin was exploited to develop a simple, sensitive assay with specificity based on a palytoxin neutralizing monoclonal antibody. Suspensions of murine erythrocytes incubated at 37°C in round-bottom microtiter trays formed visible cell pellets which could be lysed by palytoxin. Hemolysis by palytoxin was time- and temperature-dependent, with a 24 hr detection limit of 1 pg/ml. The assay selectively detected palytoxin in a crude extract of
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Bignami. A rapid and sensitive hemolysis neutralization assay for palytoxin.
Toxicon
31, 817–820, 1993.—The hemolytic action of palytoxin was exploited to develop a simple, sensitive assay with specificity based on a palytoxin neutralizing monoclonal antibody. Suspensions of murine erythrocytes incubated at 37°C in round-bottom microtiter trays formed visible cell pellets which could be lysed by palytoxin. Hemolysis by palytoxin was time- and temperature-dependent, with a 24 hr detection limit of 1 pg/ml. The assay selectively detected palytoxin in a crude extract of
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Bignami. A rapid and sensitive hemolysis neutralization assay for palytoxin.
Toxicon
31, 817–820, 1993.—The hemolytic action of palytoxin was exploited to develop a simple, sensitive assay with specificity based on a palytoxin neutralizing monoclonal antibody. Suspensions of murine erythrocytes incubated at 37°C in round-bottom microtiter trays formed visible cell pellets which could be lysed by palytoxin. Hemolysis by palytoxin was time- and temperature-dependent, with a 24 hr detection limit of 1 pg/ml. The assay selectively detected palytoxin in a crude extract of
Palythoa tuberculosa.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>8102021</pmid><doi>10.1016/0041-0101(93)90389-Z</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0041-0101 |
| ispartof | Toxicon (Oxford), 1993-06, Vol.31 (6), p.817-820 |
| issn | 0041-0101 1879-3150 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16693029 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Acrylamides Animal poisons toxicology. Antivenoms Animals Antibodies, Monoclonal Biological and medical sciences Cnidarian Venoms - analysis Erythrocytes - drug effects Female Hemolysis Immunoenzyme Techniques Marine Medical sciences Mice Neutralization Tests - methods Sensitivity and Specificity Toxicology |
| title | A rapid and sensitive hemolysis neutralization assay for palytoxin |
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