A rapid and sensitive hemolysis neutralization assay for palytoxin

G. S. Bignami. A rapid and sensitive hemolysis neutralization assay for palytoxin. Toxicon 31, 817–820, 1993.—The hemolytic action of palytoxin was exploited to develop a simple, sensitive assay with specificity based on a palytoxin neutralizing monoclonal antibody. Suspensions of murine erythrocyte...

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Veröffentlicht in:	Toxicon (Oxford) 1993-06, Vol.31 (6), p.817-820
1. Verfasser:	Bignami, Gary S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acrylamides Animal poisons toxicology. Antivenoms Animals Antibodies, Monoclonal Biological and medical sciences Cnidarian Venoms - analysis Erythrocytes - drug effects Female Hemolysis Immunoenzyme Techniques Marine Medical sciences Mice Neutralization Tests - methods Sensitivity and Specificity Toxicology
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Zusammenfassung:	G. S. Bignami. A rapid and sensitive hemolysis neutralization assay for palytoxin. Toxicon 31, 817–820, 1993.—The hemolytic action of palytoxin was exploited to develop a simple, sensitive assay with specificity based on a palytoxin neutralizing monoclonal antibody. Suspensions of murine erythrocytes incubated at 37°C in round-bottom microtiter trays formed visible cell pellets which could be lysed by palytoxin. Hemolysis by palytoxin was time- and temperature-dependent, with a 24 hr detection limit of 1 pg/ml. The assay selectively detected palytoxin in a crude extract of Palythoa tuberculosa.
ISSN:	0041-0101 1879-3150
DOI:	10.1016/0041-0101(93)90389-Z