Localization of β-glucan synthetase in membranes of Paracoccidioides brasiliensis

To localize beta -1,3-glucan synthetase in membranes of Paracoccidioides brasiliensis, particulate preparations were obtained from both phases of the fungus and separated on sucrose gradients. The yeast glucan synthetase distributed between the 20% and 30% sucrose bands whereas the mycelial enzyme c...

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Veröffentlicht in:	Journal of medical and veterinary mycology 1993, Vol.31 (6), p.421-426
Hauptverfasser:	SORAIS-LANDAEZ, F, SAN-BLAS, G
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Fundamental and applied biological sciences. Psychology Growth, nutrition, metabolism, transports, enzymes. Molecular biology Microbiology Mycology Paracoccidioides brasiliensis
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description	To localize beta -1,3-glucan synthetase in membranes of Paracoccidioides brasiliensis, particulate preparations were obtained from both phases of the fungus and separated on sucrose gradients. The yeast glucan synthetase distributed between the 20% and 30% sucrose bands whereas the mycelial enzyme concentrated in the 40% band. In all cases, vanadate-sensitive, azide-insensitive ATPase was found together with glucan synthetase, suggesting that the latter is located in the cytoplasmic membrane.
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source	Taylor & Francis:Master (3349 titles); Oxford University Press Journals Digital Archive Legacy
subjects	Biological and medical sciences Fundamental and applied biological sciences. Psychology Growth, nutrition, metabolism, transports, enzymes. Molecular biology Microbiology Mycology Paracoccidioides brasiliensis
title	Localization of β-glucan synthetase in membranes of Paracoccidioides brasiliensis
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