Effect of exogenous phytohormones and sucrose on micropropagation and microtuberisation of Manihot esculenta Crantz var. TMS 96/0023

The effect of exogenous phytohormones and sucrose on micropropagation and microtuberization from nodal cuttings of Manihot esculenta was studied. When nodal cutting were cultured in the presence of 0.01 to 0.1 mg.L[sup -1] of BAP or NAA there was induction of the building of axillary buds, with the highest percentages of nodal cuttings with axillaries buds budded of 98 and 73% with 0.07 mg.L[sup -1] BAP and 0.05 mg.L[sup -1] NAA, respectively. The results of this work show that BAP, NAA, and Kin used separately or in ratio can be used for organogenesis and micropropagation in Manihot esculenta var in vitro.