Evaluation of the Performance of a Rapid Enzyme-Linked Immunosorbent Assay in the Detection of Anaplasma phagocytophilum Antibodies in Horses

The aim of this study was to evaluate the performance of a commercially available rapid enzyme-linked immonosorbent assay, the Snap ® 4Dx test, in the detection of Anaplasma phagocytophilum antibodies in horses. Two hundred apparently healthy horses (asymptomatic) and 244 animals showing clinical sy...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Vector borne and zoonotic diseases (Larchmont, N.Y.) N.Y.), 2014-05, Vol.14 (5), p.317-323
Hauptverfasser: Veronesi, Fabrizia, Passamonti, Fabrizio, Moretti, Annabella, Morganti, Giulia, Vardi, Doron Moshe, Laus, Fulvio, Marenzoni, Maria Luisa, Spaterna, Andrea, Coletti, Mauro, Piergili Fioretti, Daniela
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The aim of this study was to evaluate the performance of a commercially available rapid enzyme-linked immonosorbent assay, the Snap ® 4Dx test, in the detection of Anaplasma phagocytophilum antibodies in horses. Two hundred apparently healthy horses (asymptomatic) and 244 animals showing clinical symptoms (symptomatic), were tested for A. phagocytophilum immunoglobulin G (IgG) antibodies using both the Snap ® 4Dx kit and an indirect fluorescence antibody test (IFAT), with the latter serving as a comparative test. Horses belonging to the symptomatic group were also tested for evidence of active infection with A. phagocytophilum by analysis of IFAT IgM titers and PCR assay amplifying a specific fragment of the 16S rRNA gene. The overall agreement between the results obtained using the two tests, as well as the relative performance exhibited by the Snap ® 4Dx test in the two groups, was assessed. Forty of the 45 animals (89%) testing positive for IgG antibodies using IFAT were correctly identified using Snap ® 4Dx testing. The agreement between the results of the two tests was very high ( k >0.9), with almost identical performances in both symptomatic and asymptomatic animals. Conversely, within the symptomatic group, only 44% (no. 11/25) of Snap ® 4Dx positives appeared to be associated with a state of active infection, whereas the remaining 56% (no. 14/25) were related both to not infected animals (no. 1) and to horses whose status of infection needed further evaluations to be confirmed (no. 13/25). This study suggests that the Snap ® 4Dx test could represent a valid screening method for use during epidemiological surveys of equine populations. Nevertheless, in-clinic application of the test does not appear to be merited.
ISSN:1530-3667
1557-7759
DOI:10.1089/vbz.2013.1424