Extreme PCR: a breakthrough innovation for outbreaks?

Extreme PCR: a breakthrough innovation for outbreaks?

Annealing occurs between primers and singlestranded DNA targets. Because there are 2 oligonucleotides binding, it is a "second-order issue." [...]denaturation "is so fast that it is hard to measure and does not require any chemistry modification."

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Bibliographische Detailangaben
Veröffentlicht in:Clinical chemistry (Baltimore, Md.) Md.), 2015-04, Vol.61 (4), p.674-676
Hauptverfasser: Sheel Kumar, Vikram, Webster, Molly
Format: Artikel
Sprache:eng
Schlagworte:
Annealing
Colleges & universities
Disease Outbreaks
DNA, Viral - chemistry
DNA, Viral - genetics
Ebolavirus - genetics
Humans
Laboratories
Microfluidics - instrumentation
Microfluidics - methods
Polymerase Chain Reaction - instrumentation
Polymerase Chain Reaction - methods
Public health
Temperature
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Beschreibung
Zusammenfassung:Annealing occurs between primers and singlestranded DNA targets. Because there are 2 oligonucleotides binding, it is a "second-order issue." [...]denaturation "is so fast that it is hard to measure and does not require any chemistry modification."
ISSN:0009-9147
1530-8561
DOI:10.1373/clinchem.2014.236950