Liquid culture production of microsclerotia and submerged conidia by Trichoderma harzianum active against damping-off disease caused by Rhizoctonia solani

Liquid culture production of microsclerotia and submerged conidia by Trichoderma harzianum active against damping-off disease caused by Rhizoctonia solani

Media and culturing protocols were identified that supported the formation of submerged conidia and microsclerotia (MS) by Trichoderma harzianum Rifai strain T-22 using liquid culture fermentation. Liquid media with a higher carbon concentration (36 g L−1) promoted MS formation at all C:N ratios tes...

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Veröffentlicht in:Fungal biology 2015-04, Vol.119 (4), p.179-190
Hauptverfasser: Kobori, Nilce N., Mascarin, Gabriel M., Jackson, Mark A., Schisler, David A.
Format: Artikel
Sprache:eng
Schlagworte:
Biofungicide
C:N ratio
Carbon - metabolism
Cold Temperature
Cucurbitaceae - microbiology
Culture Media - chemistry
Desiccation
Desiccation tolerance
Hyphae - growth & development
Microbial Interactions
Microbial Viability
Nitrogen - metabolism
Pest Control, Biological
Plant Diseases - microbiology
Plant Diseases - prevention & control
Preservation, Biological
Rhizoctonia - growth & development
Sclerotia
Soilborne disease
Spores, Fungal - growth & development
Storage stability
Time Factors
Trichoderma - growth & development
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Zusammenfassung:Media and culturing protocols were identified that supported the formation of submerged conidia and microsclerotia (MS) by Trichoderma harzianum Rifai strain T-22 using liquid culture fermentation. Liquid media with a higher carbon concentration (36 g L−1) promoted MS formation at all C:N ratios tested. Hyphae aggregated to form MS after 2 d growth and after 7 d MS were fully melanized. This is the first report of MS formation by T. harzianum or any species of Trichoderma. Furthermore, submerged conidia formation was induced by liquid culture media, but yields, desiccation tolerance, and storage stability varied with C:N ratio and carbon rate. Air-dried MS granules (
ISSN:1878-6146
1878-6162
DOI:10.1016/j.funbio.2014.12.005