A new possibility for the direct use of soybean lipoxygenase on concentrated triglycerides

A soybean lipoxygenase preparation was tested to directly oxidize triglycerides. It was demonstrated that the reaction may occur without lipase intervention. The activity of the enzyme was largely stabilized by the direct use of a crude enzyme preparation. The nonpurified enzyme was able to react efficiently on complex lipids in a medium containing a low water concentration and in the absence of any surfactant or organic solvent; the activity of the enzyme was even increased when no solvent of the substrate was used.