Stability and bioactivity of gibberellic acid in different solvents

HPLC chromatograms indicated that transformation of gibberellic acid (GA3) to the corresponding dicarboxylic acid via iso-GA3 occurred in weak alkaline solution. The bioactivity of this dicarboxylic acid was about 20% that of GA3 and above 0.3 M NaOH this compound appeared to decompose. Aqueous solu...

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Veröffentlicht in:Biotechnology techniques 1993-05, Vol.7 (5), p.391-396
Hauptverfasser: Du Preez, J.C, Qian, X.M, Kilian, S.G
Format: Artikel
Sprache:eng
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Zusammenfassung:HPLC chromatograms indicated that transformation of gibberellic acid (GA3) to the corresponding dicarboxylic acid via iso-GA3 occurred in weak alkaline solution. The bioactivity of this dicarboxylic acid was about 20% that of GA3 and above 0.3 M NaOH this compound appeared to decompose. Aqueous solutions of NaOH cannot, therefore, be used as solvent in bioassays of GA3 activity.
ISSN:0951-208X
1573-6784
DOI:10.1007/BF00152547