Rapid measurement of toxicity using electrochromic dyes and frog embryos
Results are reported from studies on a 30 minute bioassay based on the use of a fluorescent dye (di-4-Anepps) to measure changes in the membrane potential across a cell, using blastula-stage embryos of the South African clawed frog (Xenopus laevis), with 6-aminonicotinamide as the toxicant. From the...
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| Veröffentlicht in: | Bulletin of environmental contamination and toxicology 1993-10, Vol.51 (4), p.557-563 |
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| container_end_page | 563 |
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| container_title | Bulletin of environmental contamination and toxicology |
| container_volume | 51 |
| creator | STRINGER, B. K BLANKEMEYER, J. T |
| description | Results are reported from studies on a 30 minute bioassay based on the use of a fluorescent dye (di-4-Anepps) to measure changes in the membrane potential across a cell, using blastula-stage embryos of the South African clawed frog (Xenopus laevis), with 6-aminonicotinamide as the toxicant. From the results, membrane-potential EC50 was predictive of the lethal effects of the toxicant rather than of its teratogenic (malformation effects). |
| doi_str_mv | 10.1007/BF00192172 |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 0007-4861 |
| ispartof | Bulletin of environmental contamination and toxicology, 1993-10, Vol.51 (4), p.557-563 |
| issn | 0007-4861 1432-0800 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Animal, plant and microbial ecology Animals Applied ecology Biological and medical sciences Ecotoxicology, biological effects of pollution Embryo, Nonmammalian - drug effects Embryo, Nonmammalian - physiology Female Fundamental and applied biological sciences. Psychology Gramicidin - toxicity Membrane Potentials - drug effects Techniques Toxicology - methods Valinomycin - toxicity Xenopus laevis Xenopus laevis - embryology |
| title | Rapid measurement of toxicity using electrochromic dyes and frog embryos |
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