Rapid measurement of toxicity using electrochromic dyes and frog embryos

Results are reported from studies on a 30 minute bioassay based on the use of a fluorescent dye (di-4-Anepps) to measure changes in the membrane potential across a cell, using blastula-stage embryos of the South African clawed frog (Xenopus laevis), with 6-aminonicotinamide as the toxicant. From the...

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Veröffentlicht in:	Bulletin of environmental contamination and toxicology 1993-10, Vol.51 (4), p.557-563
Hauptverfasser:	STRINGER, B. K, BLANKEMEYER, J. T
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal, plant and microbial ecology Animals Applied ecology Biological and medical sciences Ecotoxicology, biological effects of pollution Embryo, Nonmammalian - drug effects Embryo, Nonmammalian - physiology Female Fundamental and applied biological sciences. Psychology Gramicidin - toxicity Membrane Potentials - drug effects Techniques Toxicology - methods Valinomycin - toxicity Xenopus laevis Xenopus laevis - embryology
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Beschreibung
Zusammenfassung:	Results are reported from studies on a 30 minute bioassay based on the use of a fluorescent dye (di-4-Anepps) to measure changes in the membrane potential across a cell, using blastula-stage embryos of the South African clawed frog (Xenopus laevis), with 6-aminonicotinamide as the toxicant. From the results, membrane-potential EC50 was predictive of the lethal effects of the toxicant rather than of its teratogenic (malformation effects).
ISSN:	0007-4861 1432-0800
DOI:	10.1007/BF00192172