Immobilization of glucose isomerase on Indion 48-R

The glucose isomerase from Streptomyces sp. NCIM 2730 was produced using wheat bran or bagasse hydrolyzate as an inducer and immobilized on an inexpensive anion exchange resin Indion 48-R. The adsorption capacity and efficiency of the resin were found to be 25 U g −1 and 60%, respectively. The properties of soluble and immobilized glucose isomerase were compared. The stability of the enzyme at higher temperatures and acidic pH is increased due to immobilization. Concentrated glucose syrup (25%) was efficiently converted to fructose by the immobilized enzyme. After the seventh reuse, the conversion capacity of immobilized glucose isomerase was reduced to 61%, with a 35% loss in enzyme activity.