High-frequency (139.5 GHz) EPR spectroscopy of the tyrosyl radical in Escherichia coli ribonucleotide reductase

In this communication we report the 139.5-GHz EPR study of the stable tyrosyl radical (Y sub(122)) in Escherichia coli ribonucleoside diphosphate reductase (RDPR). It is the first high-frequency EPR spectrum of an endogenous protein radical that exhibits both Zeeman and hyperfine structure and thus...

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Veröffentlicht in:	Journal of the American Chemical Society 1993-07, Vol.115 (14), p.6420-6421
Hauptverfasser:	Gerfen, Gary J, Bellew, Brendan F, Un, Sun, Bollinger, Joseph M, Stubbe, JoAnne, Griffin, Robert G, Singel, David J
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences Enzymes and enzyme inhibitors Escherichia coli Fundamental and applied biological sciences. Psychology Oxidoreductases
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Zusammenfassung:	In this communication we report the 139.5-GHz EPR study of the stable tyrosyl radical (Y sub(122)) in Escherichia coli ribonucleoside diphosphate reductase (RDPR). It is the first high-frequency EPR spectrum of an endogenous protein radical that exhibits both Zeeman and hyperfine structure and thus provides a detailed characterization of the radical center.
ISSN:	0002-7863 1520-5126
DOI:	10.1021/ja00067a071