Monoclonal antibodies against Aeromonas salmonicida for serological diagnosis of furunculosis

Hybridomas which produce species-specific and strain-specific monoclonal antibodies (MAbs) against Aeromonas salmonicida have been established. Reaction of three MAbs, Nos. 9, 17, and 40, was strain-specific while one MAb, No.27, was species-specific. MAb 27 reacted with A. salmonicida subsp. salmonicida, achromogenes, masoucida, and atypical A. salmonicida. The titer of this MAb, a culture fluid of hybridoma, was 1:8, 1:256, 1:8, and 1:128 for agglutination test, ELISA, FAT, and colony blotting test, respectively. The molecular weight of the antigen recognized by MAb 27 was 51 to 62 kDa.