Immobilisation of bromoperoxidase from Corallina officinalis

The vanadium-dependent bromoperoxidase from the macroalga Corallina officinalis was immobilised on a cellulose acetate support with retention of approaching 50% of the applied units of activity. The enzyme exhibited high thermal stability and retained activity in repeated use. The immobilised enzyme...

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Veröffentlicht in:	Biotechnology techniques 1994-08, Vol.8 (8), p.579-582
Hauptverfasser:	Sheffield, D.J, Harry, T.R, Smith, A.J, Rogers, L.J
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology cellulose acetate Corallina officinalis enzyme activity Fundamental and applied biological sciences. Psychology immobilization Immobilization of enzymes and other molecules Immobilization techniques immobilized enzymes Methods. Procedures. Technologies peroxidases Rhodophycota
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Beschreibung
Zusammenfassung:	The vanadium-dependent bromoperoxidase from the macroalga Corallina officinalis was immobilised on a cellulose acetate support with retention of approaching 50% of the applied units of activity. The enzyme exhibited high thermal stability and retained activity in repeated use. The immobilised enzyme showed tolerance to organic solvents similar to that of the free enzyme in the case of methanol but differed for acetone and ethanol, and with the latter showed enhanced activity as the % by volume of the solvent was increased.
ISSN:	0951-208X 1573-6784
DOI:	10.1007/BF00152150