Evaluation of cassava (Manihot esculenta Crantz) germplasm collections using RAPD markers

Randomly amplified polymorphic DNA (RAPD) markers combined with the Simple Matching index for calculating the distance matrix were used to determine the genetic diversity of African cassava clones. A preliminary study of three Manihot species using 20 primers clearly showed that RAPD markers were re...

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Veröffentlicht in:	Euphytica 1993-01, Vol.74 (3), p.203-209
Hauptverfasser:	Marmey, P. (Institut de Recherche Scientifique pour le Developpement en Cooperation, Montpellier (France). Lab. de Ressources Genetiques et d'Amelioration des Plantes Tropicales), Beeching, J.R, Hamon, S, Charrier, A
Format:	Artikel
Sprache:	eng
Schlagworte:	ADN AFRICA AFRIQUE Agronomy. Soil science and plant productions Biological and medical sciences Biotechnology DNA Fundamental and applied biological sciences. Psychology Generalities. Genetics. Plant material GENETIC MARKERS Genetic resources, diversity GENETIC VARIATION Genetics and breeding of economic plants GERMOPLASMA GERMPLASM INTROGRESION INTROGRESSION ISOENZIMAS ISOENZYME ISOENZYMES MANIHOT ESCULENTA MANIHOT GLAZIOVII MARCADORES GENETICOS MARQUEUR GENETIQUE MATERIEL GENETIQUE Plant material VARIACION GENETICA VARIATION GENETIQUE
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Zusammenfassung:	Randomly amplified polymorphic DNA (RAPD) markers combined with the Simple Matching index for calculating the distance matrix were used to determine the genetic diversity of African cassava clones. A preliminary study of three Manihot species using 20 primers clearly showed that RAPD markers were relevant for analyzing their genetic diversity. DNAs from 19 cultivars of Manihot esculenta Crantz (cassava) were amplified using 8 primers. Cultivars were well discriminated and coherently distributed, comparing to previous results from isozymes and RFLP studies. Genetic diversity analysis, characterization of collections and study of introgression are the domains that RAPD markers can contribute to the improvement of cassava.
ISSN:	0014-2336 1573-5060
DOI:	10.1007/BF00040402