Binding pathway of histamine to the hH4R, observed by unconstrained molecular dynamics

[Display omitted] Histamine binds with high affinity to the human histamine H4 receptor (hH4R). We are the first to examine the complete binding pathway of histamine from the extracellular side to the orthosteric binding site of the hH4R by means of unconstrained molecular dynamic simulation. Furthermore, the simulations show that the positively charged amine moiety of the histamine interacts electrostatically with the highly conserved Asp3.32, while the imidazole moiety forms a hydrogen bond interaction with Glu5.46 and Gln7.42.