Transepithelial resistance and claudin expression in trout RTgill-W1 cell line: Effects of osmoregulatory hormones

In the present study, we examined the trout gill cell line RTgill-W1 as a possible tool for in vitro investigation of epithelial gill function in fish. After seeding in transwells, transepithelial resistance (TER) increased until reaching a plateau after 1–2days (20–80Ω⋅cm2), which was then maintain...

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Veröffentlicht in:	Comparative biochemistry and physiology. Part A, Molecular & integrative physiology Molecular & integrative physiology, 2015-04, Vol.182, p.45-52
Hauptverfasser:	Trubitt, Rebecca T., Rabeneck, D. Brett, Bujak, Joanna K., Bossus, Maryline C., Madsen, Steffen S., Tipsmark, Christian K.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Line Cinnamates - pharmacology Claudin-10e Claudin-30 Claudins - genetics Claudins - metabolism Cortisol Edetic Acid - pharmacology Epithelial Cells - drug effects Epithelial Cells - metabolism Fish Proteins - genetics Fish Proteins - metabolism Gills - cytology Gills - drug effects Gills - metabolism Growth hormone Growth Hormone - metabolism Growth Hormone - pharmacology Hormones - pharmacology Hormones - physiology Hydrocortisone - metabolism Hydrocortisone - pharmacology Na+,K+-ATPase Oncorhynchus mykiss - physiology Osmoregulation Osmoregulation - physiology Prolactin Prolactin - metabolism Prolactin - pharmacology Rainbow trout Tight junctions Tight Junctions - drug effects Tight Junctions - metabolism Zonula occludens-1 Zonula Occludens-1 Protein - genetics Zonula Occludens-1 Protein - metabolism
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Zusammenfassung:	In the present study, we examined the trout gill cell line RTgill-W1 as a possible tool for in vitro investigation of epithelial gill function in fish. After seeding in transwells, transepithelial resistance (TER) increased until reaching a plateau after 1–2days (20–80Ω⋅cm2), which was then maintained for more than 6days. Tetrabromocinnamic acid, a known stimulator of TER via casein kinase II inhibition, elevated TER in the cell line to 125% of control values after 2 and 6h. Treatment with ethylenediaminetetraacetic acid induced a decrease in TER to
ISSN:	1095-6433 1531-4332
DOI:	10.1016/j.cbpa.2014.12.005