Protective efficacy and immune responses induced by a DNA vaccine encoding codon-optimized PPA1 against Photobacterium damselae subsp. piscicida in Japanese flounder

Protective efficacy and immune responses induced by a DNA vaccine encoding codon-optimized PPA1 against Photobacterium damselae subsp. piscicida in Japanese flounder

Highlights • Protective efficacy of DNA vaccine against pseudotuberculosis was investigated. • Wild-type and codon-optimized PPA1 gene were used as an antigen for the vaccine. • The codon optimization enhanced the immunogenicity of the DNA vaccine in fish. • The codon-optimized vaccine induced stron...

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Veröffentlicht in:Vaccine 2015-02, Vol.33 (8), p.1040-1045
Hauptverfasser: Kato, Goshi, Yamashita, Kozue, Kondo, Hidehiro, Hirono, Ikuo
Format: Artikel
Sprache:eng
Schlagworte:
Agglutination Tests
Allergy and Immunology
Amino Acid Sequence
Animals
Antibodies, Bacterial - immunology
Antigens, Bacterial - chemistry
Antigens, Bacterial - genetics
Antigens, Bacterial - immunology
Bacterial pseudotuberculosis
Bacterial Vaccines - administration & dosage
Bacterial Vaccines - genetics
Bacterial Vaccines - immunology
Base Sequence
Codon optimization
Codon usage
Deoxyribonucleic acid
DNA
Fish
Fish Diseases - immunology
Fish Diseases - mortality
Fish Diseases - prevention & control
Flounder
Gene Expression
Genes
Immune system
Immunogenicity
Marine
Marine fish
Molecular Sequence Data
Pasteurella piscicida
Pasteurellosis
Photobacterium - immunology
Photobacterium damselae
Pleuronectiformes
Vaccines
Vaccines, DNA - administration & dosage
Vaccines, DNA - genetics
Vaccines, DNA - immunology
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Zusammenfassung:Highlights • Protective efficacy of DNA vaccine against pseudotuberculosis was investigated. • Wild-type and codon-optimized PPA1 gene were used as an antigen for the vaccine. • The codon optimization enhanced the immunogenicity of the DNA vaccine in fish. • The codon-optimized vaccine induced strong protection against pseudotuberculosis.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2015.01.011