Evidence of active aggregation behaviour in Lucilia sericata larvae and possible implication of a conspecific mark

Vectors of aggregation are well known for some arthropod species, but not for many others. We aimed to describe larval aggregation (experiment 1) in the carrion fly, Lucilia sericata (Diptera: Calliphoridae), and to investigate the effect of food and conspecifics on larval behaviour (experiment 2). In experiment 1, 40 larvae were placed in a petri dish with a homogeneous diet for 30min, 1h, 3h, 5h or 24h. This experiment demonstrated for the first time under controlled conditions the active aggregation of L. sericata larvae. The results indicate that the aggregation took place quickly and was reinforced with time. After only 3h, one main aggregate comprising a majority of individuals was observed. These results also highlight the likely use by necrophagous larvae of a signal left by conspecifics as an aggregation vector. In experiment 2, we used a video-tracking system to investigate whether such an aggregative signal exists. Fed and starved larvae were tracked for 5min in a circular area with each half marked with a different signal combination. The time spent in the signal zones, the distance travelled, the velocity, the time at the stop and the number of stops in each zone were measured. The larvae were significantly retained by a signal (mark) left by conspecifics. Together, the results of this study demonstrate the existence of a contact and/or odour-mediated signal involved in the aggregative behaviour of necrophagous larvae. •The aggregation took place quickly and increased with time.•Our results suggest that thigmotaxis affects larval distribution.•Our results suggest that larvae deposit a mark that is detected by the other larvae.•Larvae are able to detect and to stay in the zone where this signal is deposited.•This mark is a potential vector of aggregation in this species.