Capture and intermediate purification of recombinant antibodies with combined precipitation methods

•Combined precipitation methods with equal separation power as chromatographic methods.•Replacement of capture and intermediate steps by caprylic acid and PEG precipitation.•Combination of CEP, PEG, CA and CaCl2 precipitation to remove host cell impurities.•The final yield and purity of the antibodies nearly reached drug substance values. A protocol for capturing recombinant antibodies was developed with a combination of different precipitation methods. This process comprised four different precipitations, including caprylic acid, polyethylene glycol (PEG), CaCl2, and cold ethanol. The final yield and purity of the antibodies nearly reached drug substance values, with a low host cell protein concentration (