The in vitro immunomodulatory effect of extracellular products (ECPs) of Vagococcus fluvialis L21 on European sea bass (Dicentrarchus labrax) leucocytes

The immune associated genes, interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin 10 (IL-10), tumor necrosis factor-α (TNF-α), ciclo-oxigenase-2 (COX-2), and Mx gene were studied by real-time PCR in head-kidney leucocytes of sea bass after incubation with the extracellular products (ECPs) of th...

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Veröffentlicht in:	Fish & shellfish immunology 2015-02, Vol.42 (2), p.517-521
Hauptverfasser:	Román, L., Acosta, F., Padilla, D., El Aamri, F., Bravo, J., Vega, B., Rodriguez, E., Vega, J., Déniz, S., Real, F.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal Feed - analysis Animals Bass - genetics Bass - immunology Bass - metabolism Dicentrarchus labrax Diet - veterinary ECPs Enterococcaceae - chemistry European sea bass Fish immune system Fish Proteins - genetics Fish Proteins - metabolism Gene Expression Regulation Immunity, Innate - immunology Immunomodulation Leucocytes Leukocytes - immunology Marine Probiotics Vagococcus fluvialis Vagococcus fluvialis L21
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Zusammenfassung:	The immune associated genes, interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin 10 (IL-10), tumor necrosis factor-α (TNF-α), ciclo-oxigenase-2 (COX-2), and Mx gene were studied by real-time PCR in head-kidney leucocytes of sea bass after incubation with the extracellular products (ECPs) of the probiotic strain Vagococcus fluvialis L21 and polyinosinic:polycytidylic acid (POLY I:C), at different times (T1.5, T6, T12, T24, T48 and T72). In general, we can observe how pro-inflammatory cytokines IL-1β, TNF-α, IL-6 and COX-2 studied displayed a strong peak after stimulation with 1.5 h of ECPs of V. fluvialis L21, significant differences (P
ISSN:	1050-4648 1095-9947
DOI:	10.1016/j.fsi.2014.11.037