Effect of 5-Aza-CdR on Growth and Invasion Ability of Non-small Cell Lung Cancer A549 and Expression of TFPI-2 Gene mRNA in vitro

Objective To explore the effect of 5-aza-2-deoxycytidine(5-Aza-CdR) on the proliferation of non-small cell lung cancer cell line A549 and the expression of tissue factor pathway inhibitor 2 (TFPI-2) gene mRNA, and to explore whether 5-Aza-CdR could inhibit the invasion capacity of NSCLC A549 cell via TFPI-2 gene. Methods A549 cells were treated with 5-Aza-CdR at different concentrations. The proliferation of A549 cells was detected by MTT assay after treatment for 24 h, 48 h and 72 h, respectively. Cell cycle of A549 cells were analyzed by flow cytometer(FCM) after treatment for 72 h. Results MTT showed that A549 cell growth had been restrained after treatment at different concentrations for 24 h, 48 h and 72 h, respectively, in an obvious dose- and time-dependence manner. Conclusion 5-Aza-CdR could restore TFPI-2 gene expression in NSCLC cell line A549 cells and restrain the proliferation and invasion capacity of A549 cells by reducing TFPI-2 gene methylation.