Expression, Purification, and Characterization of Recombinant Coptotermes Formosanus Tropomyosin

Expression, Purification, and Characterization of Recombinant Coptotermes Formosanus Tropomyosin

Purified recombinant protein was analyzed by circular dichroism, mass-spectrometry, immunoblot with IgG and IgE antibodies, and was subjected to simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) digestion.

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Bibliographische Detailangaben
Veröffentlicht in:Journal of allergy and clinical immunology 2015-02, Vol.135 (2), p.AB188-AB188
Hauptverfasser: Vargas, Aurora M, Grimm, Casey C., PhD, Mattison, Christopher P., PhD
Format: Artikel
Sprache:eng
Schlagworte:
Allergy and Immunology
Pest control
Proteins
Termites
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Beschreibung
Zusammenfassung:Purified recombinant protein was analyzed by circular dichroism, mass-spectrometry, immunoblot with IgG and IgE antibodies, and was subjected to simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) digestion.
ISSN:0091-6749
1097-6825
DOI:10.1016/j.jaci.2014.12.1547