Insulinotrophic and hypolipidemic effects of Ecklonia cava in streptozotocin-induced diabetic mice

<正>Objective:To explore the anti-diabetic activity of Ecklonia cava(EC) in streptozotocin(STZ)-induced diabetic mice.Methods:Diabetes was induced by a single intraperitoneal injection of STZ(90 mg/kg).Normal and diabetic mice were treated with 0%,3%,and 5%EC diet for 4 weeks. Serum glucose and insulin concentrations,serum lipid profile,oral glucose tolerance test,and liver and pancreaticβ-cell histopathological observations were performed.In addition,in vitro glucose-induced insulin secretion was determined using pancreaticβ-islet cells.Results:EC supplementation significantly and dose-dependently decreased serum glucose concentration, and improved glucose homeostasis in diabetic mice by preventing loss ofβ-cell mass resulting in increase of insulin secretion.The triglyceride and total cholesterol concentrations in the serum and liver were markedly reduced by EC treatment in STZ-diabetic mice.Moreover,LDL-,and HDL-cholesterol levels were ameliorated in EC supplemented diabetic mice.Liver steatosis induced by STZ was ameliorated by EC supplementation.Furthermore,in vitro insulinotrophic effect of EC extract was observed in pancreaticβ-islets.Conclusions:This study demonstrated that EC is a potent and efficacious hypoglycemic and hypolipidemic agent,and prevents the loss ofβ-cell mass resulting in increase of insulin secretary capacity.