A biopharmaceutical plant on a chip: continuous micro-devices for the production of monoclonal antibodies
We report a proof-of-principle for the use of micro-devices as continuous bioreactors for the production of monoclonal antibodies. We culture CHO cells on the surface of PMMA "zigzag" channels textured with semi-spherical cavities coated with fibronectin, observing steady-state productivit...
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| Veröffentlicht in: | Lab on a chip 2013-04, Vol.13 (7), p.1243-1246 |
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| container_end_page | 1246 |
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| container_issue | 7 |
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| container_title | Lab on a chip |
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| creator | Garza-García, Lucía D Carrillo-Cocom, Leydi M Araiz-Hernández, Diana Soto-Vázquez, Pedro López-Meza, Julián Tapia-Mejía, Eduardo J Camacho-León, Sergio García-López, Erika Rodríguez-González, Ciro A Alvarez, Mario M |
| description | We report a proof-of-principle for the use of micro-devices as continuous bioreactors for the production of monoclonal antibodies. We culture CHO cells on the surface of PMMA "zigzag" channels textured with semi-spherical cavities coated with fibronectin, observing steady-state productivities 100 times higher than those observed in full scale systems. |
| doi_str_mv | 10.1039/c3lc50104c |
| format | Article |
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Carrillo-Cocom, Leydi M ; Araiz-Hernández, Diana ; Soto-Vázquez, Pedro ; López-Meza, Julián ; Tapia-Mejía, Eduardo J ; Camacho-León, Sergio ; García-López, Erika ; Rodríguez-González, Ciro A ; Alvarez, Mario M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-3bfde8f70788c283619a9cf59c72deb0616f21880e62d6c812b41452e2f14a323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - biosynthesis</topic><topic>Channels</topic><topic>Chips</topic><topic>CHO Cells</topic><topic>Cricetinae</topic><topic>Cricetulus</topic><topic>Culture</topic><topic>Fibronectin</topic><topic>Holes</topic><topic>Lab-On-A-Chip Devices</topic><topic>Monoclonal antibodies</topic><topic>Polymethyl Methacrylate - chemistry</topic><topic>Polymethyl methacrylates</topic><topic>Productivity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Garza-García, Lucía D</creatorcontrib><creatorcontrib>Carrillo-Cocom, Leydi M</creatorcontrib><creatorcontrib>Araiz-Hernández, Diana</creatorcontrib><creatorcontrib>Soto-Vázquez, Pedro</creatorcontrib><creatorcontrib>López-Meza, Julián</creatorcontrib><creatorcontrib>Tapia-Mejía, Eduardo J</creatorcontrib><creatorcontrib>Camacho-León, Sergio</creatorcontrib><creatorcontrib>García-López, Erika</creatorcontrib><creatorcontrib>Rodríguez-González, Ciro A</creatorcontrib><creatorcontrib>Alvarez, Mario M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Lab on a chip</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Garza-García, Lucía D</au><au>Carrillo-Cocom, Leydi M</au><au>Araiz-Hernández, Diana</au><au>Soto-Vázquez, Pedro</au><au>López-Meza, Julián</au><au>Tapia-Mejía, Eduardo J</au><au>Camacho-León, Sergio</au><au>García-López, Erika</au><au>Rodríguez-González, Ciro A</au><au>Alvarez, Mario M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A biopharmaceutical plant on a chip: continuous micro-devices for the production of monoclonal antibodies</atitle><jtitle>Lab on a chip</jtitle><addtitle>Lab Chip</addtitle><date>2013-04-07</date><risdate>2013</risdate><volume>13</volume><issue>7</issue><spage>1243</spage><epage>1246</epage><pages>1243-1246</pages><issn>1473-0197</issn><eissn>1473-0189</eissn><abstract>We report a proof-of-principle for the use of micro-devices as continuous bioreactors for the production of monoclonal antibodies. 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| fulltext | fulltext |
| identifier | ISSN: 1473-0197 |
| ispartof | Lab on a chip, 2013-04, Vol.13 (7), p.1243-1246 |
| issn | 1473-0197 1473-0189 |
| language | eng |
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| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Animals Antibodies, Monoclonal - biosynthesis Channels Chips CHO Cells Cricetinae Cricetulus Culture Fibronectin Holes Lab-On-A-Chip Devices Monoclonal antibodies Polymethyl Methacrylate - chemistry Polymethyl methacrylates Productivity |
| title | A biopharmaceutical plant on a chip: continuous micro-devices for the production of monoclonal antibodies |
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