A biopharmaceutical plant on a chip: continuous micro-devices for the production of monoclonal antibodies

We report a proof-of-principle for the use of micro-devices as continuous bioreactors for the production of monoclonal antibodies. We culture CHO cells on the surface of PMMA "zigzag" channels textured with semi-spherical cavities coated with fibronectin, observing steady-state productivit...

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Veröffentlicht in:	Lab on a chip 2013-04, Vol.13 (7), p.1243-1246
Hauptverfasser:	Garza-García, Lucía D, Carrillo-Cocom, Leydi M, Araiz-Hernández, Diana, Soto-Vázquez, Pedro, López-Meza, Julián, Tapia-Mejía, Eduardo J, Camacho-León, Sergio, García-López, Erika, Rodríguez-González, Ciro A, Alvarez, Mario M
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal - biosynthesis Channels Chips CHO Cells Cricetinae Cricetulus Culture Fibronectin Holes Lab-On-A-Chip Devices Monoclonal antibodies Polymethyl Methacrylate - chemistry Polymethyl methacrylates Productivity
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Beschreibung
Zusammenfassung:	We report a proof-of-principle for the use of micro-devices as continuous bioreactors for the production of monoclonal antibodies. We culture CHO cells on the surface of PMMA "zigzag" channels textured with semi-spherical cavities coated with fibronectin, observing steady-state productivities 100 times higher than those observed in full scale systems.
ISSN:	1473-0197 1473-0189
DOI:	10.1039/c3lc50104c