Patterned Surface Activation of Cyclo-Olefin Polymers for Biochip Applications

Two different surface activation methods (UV/ozone and oxygen plasma treatment) were applied for patterned surface activation of cyclo-olefin polymer (COP) surfaces combined with different masking techniques (metal shadow mask and protective tape). Surface properties were characterized by various methods such as contact angle measurement, ATR-IR, XPS and Surface enhanced ellipsometric contrast (SEEC) microscopy. UV/ozone and oxygen plasma allowed for patterned surface modification of COP leading to the formation of carboxylic and hydroxyl groups on the activated part of the surface. Stability against organic solvents was determined by rinsing the activated substrates with 2-propanol. For UV/ozone treatment it was found that a thin film of degradation products remains on the COP surface and is at least partly removed in the following washing or rinsing steps.