Optimization of Os-Complex Modified Redox Polymers for Improving Biocatalysis of PQQ-sGDH Based Electrodes

A variety of Os‐complex modified redox polymers were synthesized and optimized for an improved bioelectrochemical communication with PQQ‐sGDH. The properties of the polymers were varied by changing the monomers composition leading to improved immobilization and increased enzyme loading. Three Os‐complexes were designed exhibiting different ligands but similar redox potentials adapted for efficient electron transfer with PQQ‐sGDH. Effects of the ligand sphere, polymer backbone and length of the tether chain between Os‐complex and polymer backbone as well as the impact of different bifunctional crosslinkers on the catalytic current were evaluated. The optimized bioelectrodes exhibited significantly improved catalytic currents and stability.