Circuit modification in electrical field flow fractionation systems generating higher resolution separation of nanoparticles

•For the first time in ElFFF literature, electrical circuit of the system is modified.•By modifying the circuit, baseline separations of sub 50nm particles is achieved.•Separations became possible at high frequencies, which allows shorter FFF channels.•Separations achieved with low voltages reducing the risk of electrolytic breakdown.•Baseline separations became possible with nonsquare voltage waveforms as well. Compared to other sub-techniques of field flow fractionation (FFF), cyclical electrical field flow fractionation (CyElFFF) is a relatively new method with many opportunities remaining for improvement. One of the most important limitations of this method is the separation of particles smaller than 100nm. For such small particles, the diffusion rate becomes very high, resulting in severe reductions in the CyElFFF separation efficiency. To address this limitation, we modified the electrical circuitry of the ElFFF system. In all earlier ElFFF reports, electrical power sources have been directly connected to the ElFFF channel electrodes, and no alteration has been made in the electrical circuitry of the system. In this work, by using discrete electrical components, such as resistors and diodes, we improved the effective electric field in the system to allow high resolution separations. By modifying the electrical circuitry of the ElFFF system, high resolution separations of 15 and 40nm gold nanoparticles were achieved. The effects of applying different frequencies, amplitudes and voltage shapes have been investigated and analyzed through experiments.