Dynamic Structures of DNA Heptamers with Different Central Trinucleotide Sequences Studied by Electrochemical and Spectral Methods
A short, single‐stranded DNA fragment d(GCGAAGC) and its analogs d(GCNNNGC) carrying different trinucleotide sequences in the center of the molecule (AAA,CCC, GGG or TTT) were investigated by spectral (CD and UV spectra) and electrochemical methods (voltammetry, polyacrylamide gel electrophoresis)....
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| Veröffentlicht in: | Electroanalysis (New York, N.Y.) N.Y.), 2014-10, Vol.26 (10), p.2118-2128 |
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| container_title | Electroanalysis (New York, N.Y.) |
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| creator | Pilarova, Iveta Kejnovska, Iva Vorlickova, Michaela Trnkova, Libuse |
| description | A short, single‐stranded DNA fragment d(GCGAAGC) and its analogs d(GCNNNGC) carrying different trinucleotide sequences in the center of the molecule (AAA,CCC, GGG or TTT) were investigated by spectral (CD and UV spectra) and electrochemical methods (voltammetry, polyacrylamide gel electrophoresis). Our results show that (i) the central triplet GAA or AAA dramatically stabilizes DNA hairpins, (ii) a stem‐loop configuration is not supported by CCC or TTT sequences, instead, these heptamers adopt bimolecular duplex forms. Finally (iii), in the case of GGG sequence a very stable supramolecular G‐quadruplex is formed. |
| doi_str_mv | 10.1002/elan.201400288 |
| format | Article |
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Our results show that (i) the central triplet GAA or AAA dramatically stabilizes DNA hairpins, (ii) a stem‐loop configuration is not supported by CCC or TTT sequences, instead, these heptamers adopt bimolecular duplex forms. Finally (iii), in the case of GGG sequence a very stable supramolecular G‐quadruplex is formed.</description><identifier>ISSN: 1040-0397</identifier><identifier>EISSN: 1521-4109</identifier><identifier>DOI: 10.1002/elan.201400288</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>Circular dichroism ; Deoxyribonucleic acid ; Dichroism ; DNA heptamers ; Dynamic structural analysis ; Elimination voltammetric procedure ; Gene sequencing ; Polyacrylamide gel electrophoresis ; Polyacrylamides ; Spectra ; Spectral methods ; Voltammetry</subject><ispartof>Electroanalysis (New York, N.Y.), 2014-10, Vol.26 (10), p.2118-2128</ispartof><rights>2014 WILEY‐VCH Verlag GmbH & Co. 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Our results show that (i) the central triplet GAA or AAA dramatically stabilizes DNA hairpins, (ii) a stem‐loop configuration is not supported by CCC or TTT sequences, instead, these heptamers adopt bimolecular duplex forms. Finally (iii), in the case of GGG sequence a very stable supramolecular G‐quadruplex is formed.</description><subject>Circular dichroism</subject><subject>Deoxyribonucleic acid</subject><subject>Dichroism</subject><subject>DNA heptamers</subject><subject>Dynamic structural analysis</subject><subject>Elimination voltammetric procedure</subject><subject>Gene sequencing</subject><subject>Polyacrylamide gel electrophoresis</subject><subject>Polyacrylamides</subject><subject>Spectra</subject><subject>Spectral methods</subject><subject>Voltammetry</subject><issn>1040-0397</issn><issn>1521-4109</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNqNkU1v2zAMho1iA9p1ve6s4y7OKOvD1jFI0rRAmgFLh-4myDKNqHXsTJLR5rpfXgUpit22iygS70MSfLPsC4UJBSi-YWf6SQGUp6SqzrILKgqacwrqQ_oDhxyYKs-zTyE8AoCSXF1kf-aH3uycJZvoRxtHj4EMLZmvp-QG99Hs0Afy7OKWzF3bosc-kll6vOnIvXf9aDscomuQbPD3iL1N_CaOjcOG1Aey6NBGP9gtphkJMX1DNvtjLSV3GLdDEz5nH1vTBbx6i5fZz-vF_ewmX31f3s6mq9wyxarcWCOwqQRXBlRZWqnQlhJqpWpac0EpE1I0hawUcOQFNEjrqhWSFi0UDCS7zL6e-u79kFYNUe9csNils-EwBk2lBOAVF_Af0kIxKWWpknRyklo_hOCx1XvvdsYfNAV99EUffdHvviRAnYBn1-HhH2q9WE3Xf7P5iXUh4ss7a_yTliUrhX5YL7Vc_oC5uF7qX-wVw_-hRQ</recordid><startdate>201410</startdate><enddate>201410</enddate><creator>Pilarova, Iveta</creator><creator>Kejnovska, Iva</creator><creator>Vorlickova, Michaela</creator><creator>Trnkova, Libuse</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><scope>BSCLL</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope></search><sort><creationdate>201410</creationdate><title>Dynamic Structures of DNA Heptamers with Different Central Trinucleotide Sequences Studied by Electrochemical and Spectral Methods</title><author>Pilarova, Iveta ; Kejnovska, Iva ; Vorlickova, Michaela ; Trnkova, Libuse</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3938-aca5ed8549a0977c69ec760b99b1b45113565d268904e420de1b8f5612f023063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Circular dichroism</topic><topic>Deoxyribonucleic acid</topic><topic>Dichroism</topic><topic>DNA heptamers</topic><topic>Dynamic structural analysis</topic><topic>Elimination voltammetric procedure</topic><topic>Gene sequencing</topic><topic>Polyacrylamide gel electrophoresis</topic><topic>Polyacrylamides</topic><topic>Spectra</topic><topic>Spectral methods</topic><topic>Voltammetry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pilarova, Iveta</creatorcontrib><creatorcontrib>Kejnovska, Iva</creatorcontrib><creatorcontrib>Vorlickova, Michaela</creatorcontrib><creatorcontrib>Trnkova, Libuse</creatorcontrib><collection>Istex</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Electroanalysis (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pilarova, Iveta</au><au>Kejnovska, Iva</au><au>Vorlickova, Michaela</au><au>Trnkova, Libuse</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dynamic Structures of DNA Heptamers with Different Central Trinucleotide Sequences Studied by Electrochemical and Spectral Methods</atitle><jtitle>Electroanalysis (New York, N.Y.)</jtitle><addtitle>Electroanalysis</addtitle><date>2014-10</date><risdate>2014</risdate><volume>26</volume><issue>10</issue><spage>2118</spage><epage>2128</epage><pages>2118-2128</pages><issn>1040-0397</issn><eissn>1521-4109</eissn><abstract>A short, single‐stranded DNA fragment d(GCGAAGC) and its analogs d(GCNNNGC) carrying different trinucleotide sequences in the center of the molecule (AAA,CCC, GGG or TTT) were investigated by spectral (CD and UV spectra) and electrochemical methods (voltammetry, polyacrylamide gel electrophoresis). Our results show that (i) the central triplet GAA or AAA dramatically stabilizes DNA hairpins, (ii) a stem‐loop configuration is not supported by CCC or TTT sequences, instead, these heptamers adopt bimolecular duplex forms. Finally (iii), in the case of GGG sequence a very stable supramolecular G‐quadruplex is formed.</abstract><cop>Weinheim</cop><pub>WILEY-VCH Verlag</pub><doi>10.1002/elan.201400288</doi><tpages>11</tpages></addata></record> |
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| subjects | Circular dichroism Deoxyribonucleic acid Dichroism DNA heptamers Dynamic structural analysis Elimination voltammetric procedure Gene sequencing Polyacrylamide gel electrophoresis Polyacrylamides Spectra Spectral methods Voltammetry |
| title | Dynamic Structures of DNA Heptamers with Different Central Trinucleotide Sequences Studied by Electrochemical and Spectral Methods |
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