Dynamic Structures of DNA Heptamers with Different Central Trinucleotide Sequences Studied by Electrochemical and Spectral Methods

Dynamic Structures of DNA Heptamers with Different Central Trinucleotide Sequences Studied by Electrochemical and Spectral Methods

A short, single‐stranded DNA fragment d(GCGAAGC) and its analogs d(GCNNNGC) carrying different trinucleotide sequences in the center of the molecule (AAA,CCC, GGG or TTT) were investigated by spectral (CD and UV spectra) and electrochemical methods (voltammetry, polyacrylamide gel electrophoresis)....

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Veröffentlicht in:Electroanalysis (New York, N.Y.) N.Y.), 2014-10, Vol.26 (10), p.2118-2128
Hauptverfasser: Pilarova, Iveta, Kejnovska, Iva, Vorlickova, Michaela, Trnkova, Libuse
Format: Artikel
Sprache:eng
Schlagworte:
Circular dichroism
Deoxyribonucleic acid
Dichroism
DNA heptamers
Dynamic structural analysis
Elimination voltammetric procedure
Gene sequencing
Polyacrylamide gel electrophoresis
Polyacrylamides
Spectra
Spectral methods
Voltammetry
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Zusammenfassung:A short, single‐stranded DNA fragment d(GCGAAGC) and its analogs d(GCNNNGC) carrying different trinucleotide sequences in the center of the molecule (AAA,CCC, GGG or TTT) were investigated by spectral (CD and UV spectra) and electrochemical methods (voltammetry, polyacrylamide gel electrophoresis). Our results show that (i) the central triplet GAA or AAA dramatically stabilizes DNA hairpins, (ii) a stem‐loop configuration is not supported by CCC or TTT sequences, instead, these heptamers adopt bimolecular duplex forms. Finally (iii), in the case of GGG sequence a very stable supramolecular G‐quadruplex is formed.
ISSN:1040-0397
1521-4109
DOI:10.1002/elan.201400288