Flow-injection amperometric system for enzyme immunoassay

A flor-injection amperometric system was developed for monitoring the results of enzyme immunoassay. The operation of the flow-through amperometric detector is described by a limiting current equation for the working wire electrode. The possibility fo the optimization of the geometry and hydrodynamic properties of amperometric detectors in flow systems was demonstrated. The flow system was tested in the determination of peroxidase and immunoglobulin E in blood serum. Peroxidase (marker enzyme) was determined in the concentration range 5 × 10 −12–4 × 10 −10 M. The detection limit for immunoglobulin E in blood serum was 3.1 ng ml −1. The sample throughput was 400 h −1.