Crystallization and preliminary X-ray diffraction analysis of the catalytic domain of Cex, an exo-β-1,4-glucanase and β-1,4-xylanase from the bacterium Cellulomonas fimi
Single crystals of the catalytic domain of Cex, an exo-β-1,4-glucanase and β-1,4-xylanase from the cellulolytic bacterium Cellulomonas fimi, have been grown in the presence of polyethylene glycol 4000 using the vapour diffusion technique. The crystals, which diffract to better than 2.0 Å resolution,...
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Veröffentlicht in: | Journal of molecular biology 1992-11, Vol.228 (2), p.693-695 |
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Hauptverfasser: | , , , , , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Single crystals of the catalytic domain of Cex, an exo-β-1,4-glucanase and β-1,4-xylanase from the cellulolytic bacterium
Cellulomonas fimi, have been grown in the presence of polyethylene glycol 4000 using the vapour diffusion technique. The crystals, which diffract to better than 2.0 Å resolution, belong to space group
P4
12
12 or
P4
32
12 and have cell constants:
a = b = 88.21
A
̊
, c = 81.10
A
̊
; α = β = γ = 90 °. |
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ISSN: | 0022-2836 1089-8638 |
DOI: | 10.1016/0022-2836(92)90852-B |