Crystallization and preliminary X-ray diffraction analysis of the catalytic domain of Cex, an exo-β-1,4-glucanase and β-1,4-xylanase from the bacterium Cellulomonas fimi

Single crystals of the catalytic domain of Cex, an exo-β-1,4-glucanase and β-1,4-xylanase from the cellulolytic bacterium Cellulomonas fimi, have been grown in the presence of polyethylene glycol 4000 using the vapour diffusion technique. The crystals, which diffract to better than 2.0 Å resolution,...

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Veröffentlicht in:Journal of molecular biology 1992-11, Vol.228 (2), p.693-695
Hauptverfasser: Bedarkar, Sudhir, Gilkes, Neil R., Kilburn, Douglas G., Kwan, Emily, Rose, David R., Miller, Robert C., Warren, R.Antony J., Withers, Stephen G.
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Sprache:eng
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Zusammenfassung:Single crystals of the catalytic domain of Cex, an exo-β-1,4-glucanase and β-1,4-xylanase from the cellulolytic bacterium Cellulomonas fimi, have been grown in the presence of polyethylene glycol 4000 using the vapour diffusion technique. The crystals, which diffract to better than 2.0 Å resolution, belong to space group P4 12 12 or P4 32 12 and have cell constants: a = b = 88.21 A ̊ , c = 81.10 A ̊ ; α = β = γ = 90 °.
ISSN:0022-2836
1089-8638
DOI:10.1016/0022-2836(92)90852-B