Optimization of alpha-amylase and glucoamylase production by recombinant strains of Saccharomyces cerevisiae
Replacement of the regulatory sequence of the Bacillus amyloliquefaciens alpha-amylase gene (AMY1) by the yeast alcohol dehydrogenase gene promoter (ADC1p) resulted in increased levels of extracellular alpha-amylase production in Saccharomyces cerevisiae. Negative regulation of glucoamylase synthesi...
Gespeichert in:
| Veröffentlicht in: | Biotechnology letters 1994-07, Vol.16 (7), p.727-732 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 732 |
|---|---|
| container_issue | 7 |
| container_start_page | 727 |
| container_title | Biotechnology letters |
| container_volume | 16 |
| creator | D'Aguanno, V.S Pretorius, I.S |
| description | Replacement of the regulatory sequence of the Bacillus amyloliquefaciens alpha-amylase gene (AMY1) by the yeast alcohol dehydrogenase gene promoter (ADC1p) resulted in increased levels of extracellular alpha-amylase production in Saccharomyces cerevisiae. Negative regulation of glucoamylase synthesis by the STA10-encoded repressor was alleviated by replacing the native STA2 gene promoter from S. cerevisiae var. diastaticus with ADC1p. Enhanced degradation of starch was achieved when the modified versions of the AMY1 and STA2 genes were introduced jointly into S. cerevisiae. |
| doi_str_mv | 10.1007/BF00136479 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pasca</sourceid><recordid>TN_cdi_proquest_miscellaneous_16585343</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>16585343</sourcerecordid><originalsourceid>FETCH-LOGICAL-f237t-d401750c22487c377b73fd5d629a5f84c0fa6cd7257c33e18f38b485e928f9b13</originalsourceid><addsrcrecordid>eNo10E1Lw0AQBuBFFKzVi3_AHMRbdD-zm6MWq0Khh9pzmGx225UkG3cTIf56o62ngZmHl-FF6Jrge4KxfHhaYkxYxmV-gmZESJZmUmanaIYJJ6ngOT1HFzF-YIxzieUM1euud437ht75NvE2gbrbQwrNWEM0CbRVsqsH7f8XXfDVoP9wOSbBaN-UroW2T2IfwLXxN2MDWu8h-GbUJibaBPPlogNzic4s1NFcHeccbZfP74vXdLV-eVs8rlJLmezTimMiBdaUciU1k7KUzFaiymgOwiqusYVMV5KK6coMUZapkithcqpsXhI2R3eH3Onbz8HEvmhc1KauoTV-iAXJhBKMswneHiFEDbUN0GoXiy64BsJYcJJTotTEbg7Mgi9gFyay3dCp6KnVnEtO2Q_H03JX</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16585343</pqid></control><display><type>article</type><title>Optimization of alpha-amylase and glucoamylase production by recombinant strains of Saccharomyces cerevisiae</title><source>SpringerLink Journals - AutoHoldings</source><creator>D'Aguanno, V.S ; Pretorius, I.S</creator><creatorcontrib>D'Aguanno, V.S ; Pretorius, I.S</creatorcontrib><description>Replacement of the regulatory sequence of the Bacillus amyloliquefaciens alpha-amylase gene (AMY1) by the yeast alcohol dehydrogenase gene promoter (ADC1p) resulted in increased levels of extracellular alpha-amylase production in Saccharomyces cerevisiae. Negative regulation of glucoamylase synthesis by the STA10-encoded repressor was alleviated by replacing the native STA2 gene promoter from S. cerevisiae var. diastaticus with ADC1p. Enhanced degradation of starch was achieved when the modified versions of the AMY1 and STA2 genes were introduced jointly into S. cerevisiae.</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1007/BF00136479</identifier><identifier>CODEN: BILED3</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>alcohol dehydrogenase ; alpha-amylase ; Biological and medical sciences ; biological production ; Biotechnology ; Enzyme engineering ; Fermentation ; Fundamental and applied biological sciences. Psychology ; genetic recombination ; genetic regulation ; genetic transformation ; glucan 1,4-alpha-glucosidase ; Methods. Procedures. Technologies ; optimization ; Production of selected enzymes ; promoter regions ; Saccharomyces cerevisiae ; saccharomyces cerevisiae var. diastaticus ; structural genes</subject><ispartof>Biotechnology letters, 1994-07, Vol.16 (7), p.727-732</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27907,27908</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4192188$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>D'Aguanno, V.S</creatorcontrib><creatorcontrib>Pretorius, I.S</creatorcontrib><title>Optimization of alpha-amylase and glucoamylase production by recombinant strains of Saccharomyces cerevisiae</title><title>Biotechnology letters</title><description>Replacement of the regulatory sequence of the Bacillus amyloliquefaciens alpha-amylase gene (AMY1) by the yeast alcohol dehydrogenase gene promoter (ADC1p) resulted in increased levels of extracellular alpha-amylase production in Saccharomyces cerevisiae. Negative regulation of glucoamylase synthesis by the STA10-encoded repressor was alleviated by replacing the native STA2 gene promoter from S. cerevisiae var. diastaticus with ADC1p. Enhanced degradation of starch was achieved when the modified versions of the AMY1 and STA2 genes were introduced jointly into S. cerevisiae.</description><subject>alcohol dehydrogenase</subject><subject>alpha-amylase</subject><subject>Biological and medical sciences</subject><subject>biological production</subject><subject>Biotechnology</subject><subject>Enzyme engineering</subject><subject>Fermentation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genetic recombination</subject><subject>genetic regulation</subject><subject>genetic transformation</subject><subject>glucan 1,4-alpha-glucosidase</subject><subject>Methods. Procedures. Technologies</subject><subject>optimization</subject><subject>Production of selected enzymes</subject><subject>promoter regions</subject><subject>Saccharomyces cerevisiae</subject><subject>saccharomyces cerevisiae var. diastaticus</subject><subject>structural genes</subject><issn>0141-5492</issn><issn>1573-6776</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><recordid>eNo10E1Lw0AQBuBFFKzVi3_AHMRbdD-zm6MWq0Khh9pzmGx225UkG3cTIf56o62ngZmHl-FF6Jrge4KxfHhaYkxYxmV-gmZESJZmUmanaIYJJ6ngOT1HFzF-YIxzieUM1euud437ht75NvE2gbrbQwrNWEM0CbRVsqsH7f8XXfDVoP9wOSbBaN-UroW2T2IfwLXxN2MDWu8h-GbUJibaBPPlogNzic4s1NFcHeccbZfP74vXdLV-eVs8rlJLmezTimMiBdaUciU1k7KUzFaiymgOwiqusYVMV5KK6coMUZapkithcqpsXhI2R3eH3Onbz8HEvmhc1KauoTV-iAXJhBKMswneHiFEDbUN0GoXiy64BsJYcJJTotTEbg7Mgi9gFyay3dCp6KnVnEtO2Q_H03JX</recordid><startdate>19940701</startdate><enddate>19940701</enddate><creator>D'Aguanno, V.S</creator><creator>Pretorius, I.S</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>19940701</creationdate><title>Optimization of alpha-amylase and glucoamylase production by recombinant strains of Saccharomyces cerevisiae</title><author>D'Aguanno, V.S ; Pretorius, I.S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f237t-d401750c22487c377b73fd5d629a5f84c0fa6cd7257c33e18f38b485e928f9b13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>alcohol dehydrogenase</topic><topic>alpha-amylase</topic><topic>Biological and medical sciences</topic><topic>biological production</topic><topic>Biotechnology</topic><topic>Enzyme engineering</topic><topic>Fermentation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genetic recombination</topic><topic>genetic regulation</topic><topic>genetic transformation</topic><topic>glucan 1,4-alpha-glucosidase</topic><topic>Methods. Procedures. Technologies</topic><topic>optimization</topic><topic>Production of selected enzymes</topic><topic>promoter regions</topic><topic>Saccharomyces cerevisiae</topic><topic>saccharomyces cerevisiae var. diastaticus</topic><topic>structural genes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>D'Aguanno, V.S</creatorcontrib><creatorcontrib>Pretorius, I.S</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biotechnology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>D'Aguanno, V.S</au><au>Pretorius, I.S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optimization of alpha-amylase and glucoamylase production by recombinant strains of Saccharomyces cerevisiae</atitle><jtitle>Biotechnology letters</jtitle><date>1994-07-01</date><risdate>1994</risdate><volume>16</volume><issue>7</issue><spage>727</spage><epage>732</epage><pages>727-732</pages><issn>0141-5492</issn><eissn>1573-6776</eissn><coden>BILED3</coden><abstract>Replacement of the regulatory sequence of the Bacillus amyloliquefaciens alpha-amylase gene (AMY1) by the yeast alcohol dehydrogenase gene promoter (ADC1p) resulted in increased levels of extracellular alpha-amylase production in Saccharomyces cerevisiae. Negative regulation of glucoamylase synthesis by the STA10-encoded repressor was alleviated by replacing the native STA2 gene promoter from S. cerevisiae var. diastaticus with ADC1p. Enhanced degradation of starch was achieved when the modified versions of the AMY1 and STA2 genes were introduced jointly into S. cerevisiae.</abstract><cop>Dordrecht</cop><pub>Springer</pub><doi>10.1007/BF00136479</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0141-5492 |
| ispartof | Biotechnology letters, 1994-07, Vol.16 (7), p.727-732 |
| issn | 0141-5492 1573-6776 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16585343 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | alcohol dehydrogenase alpha-amylase Biological and medical sciences biological production Biotechnology Enzyme engineering Fermentation Fundamental and applied biological sciences. Psychology genetic recombination genetic regulation genetic transformation glucan 1,4-alpha-glucosidase Methods. Procedures. Technologies optimization Production of selected enzymes promoter regions Saccharomyces cerevisiae saccharomyces cerevisiae var. diastaticus structural genes |
| title | Optimization of alpha-amylase and glucoamylase production by recombinant strains of Saccharomyces cerevisiae |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T03%3A03%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pasca&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Optimization%20of%20alpha-amylase%20and%20glucoamylase%20production%20by%20recombinant%20strains%20of%20Saccharomyces%20cerevisiae&rft.jtitle=Biotechnology%20letters&rft.au=D'Aguanno,%20V.S&rft.date=1994-07-01&rft.volume=16&rft.issue=7&rft.spage=727&rft.epage=732&rft.pages=727-732&rft.issn=0141-5492&rft.eissn=1573-6776&rft.coden=BILED3&rft_id=info:doi/10.1007/BF00136479&rft_dat=%3Cproquest_pasca%3E16585343%3C/proquest_pasca%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16585343&rft_id=info:pmid/&rfr_iscdi=true |