Optimization of alpha-amylase and glucoamylase production by recombinant strains of Saccharomyces cerevisiae

Replacement of the regulatory sequence of the Bacillus amyloliquefaciens alpha-amylase gene (AMY1) by the yeast alcohol dehydrogenase gene promoter (ADC1p) resulted in increased levels of extracellular alpha-amylase production in Saccharomyces cerevisiae. Negative regulation of glucoamylase synthesi...

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Veröffentlicht in:	Biotechnology letters 1994-07, Vol.16 (7), p.727-732
Hauptverfasser:	D'Aguanno, V.S, Pretorius, I.S
Format:	Artikel
Sprache:	eng
Schlagworte:	alcohol dehydrogenase alpha-amylase Biological and medical sciences biological production Biotechnology Enzyme engineering Fermentation Fundamental and applied biological sciences. Psychology genetic recombination genetic regulation genetic transformation glucan 1,4-alpha-glucosidase Methods. Procedures. Technologies optimization Production of selected enzymes promoter regions Saccharomyces cerevisiae saccharomyces cerevisiae var. diastaticus structural genes
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Zusammenfassung:	Replacement of the regulatory sequence of the Bacillus amyloliquefaciens alpha-amylase gene (AMY1) by the yeast alcohol dehydrogenase gene promoter (ADC1p) resulted in increased levels of extracellular alpha-amylase production in Saccharomyces cerevisiae. Negative regulation of glucoamylase synthesis by the STA10-encoded repressor was alleviated by replacing the native STA2 gene promoter from S. cerevisiae var. diastaticus with ADC1p. Enhanced degradation of starch was achieved when the modified versions of the AMY1 and STA2 genes were introduced jointly into S. cerevisiae.
ISSN:	0141-5492 1573-6776
DOI:	10.1007/BF00136479