Novel Nanomonitor ultra-sensitive detection of troponin T

Troponin is the preferred biomarker for diagnosing myocardial infarction. Point of care devices have not matched the sensitivity of laboratory-based methods for measuring troponin. The Nanomonitor is a novel point-of-care device that uses the change in electrical impedance that occurs when a biomark...

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Veröffentlicht in:Clinica chimica acta 2015-03, Vol.442, p.96-101
Hauptverfasser: Barrett, Thomas W., Radha Shanmugam, Nandhinee, Selvam, Anjan Panneer, Kazmierczak, Steven C., Prasad, Shalini
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Sprache:eng
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Zusammenfassung:Troponin is the preferred biomarker for diagnosing myocardial infarction. Point of care devices have not matched the sensitivity of laboratory-based methods for measuring troponin. The Nanomonitor is a novel point-of-care device that uses the change in electrical impedance that occurs when a biomarker binds to its antibody, which is then correlated to the concentration of the target biomarker. Performance characteristics of the Nanomonitor were evaluated and compared to a standard laboratory-based method. The limit of detection of the Nanomonior for troponin T was 0.0088ng/l. Total imprecission was 2.38% and 0.85% at troponin T concentrations of 73ng/l and 1800ng/l. The functional sensitivity (10% coeffecient of variation) was 0.329ng/l. The linear regression had a slope of 0.996 (95% confidence interval, 0.991, 1.002), r=1.00, and an intercept of 15.88ng/l (95% confidence interval, −68.39ng/l, 100.15ng/l). The mean difference between the assays was −7.54ng/l, determined by Bland–Altman analysis. The Nanomonitor preliminary results have favorable performance characteristics for detecting troponin T in patient blood, provide results in 15min, and are portable. More research is needed. •We describe a novel nanotechnology device for measuring troponin T.•The Nanomonitor LOD is 0.0088 ng/L and 10% CV is 0.329 ng/L.•Total imprecision was < 3% for troponin T concentrations of 73 ng/L and 1800 ng/L.•The Nanomonitor has favorable preliminary charachteristics and detects troponin T in patient blood.•The Nanomonitor provides results in 15 min and is portable.
ISSN:0009-8981
1873-3492
DOI:10.1016/j.cca.2015.01.013