Mixed Carbonates as Useful Substrates for a Fluorogenic Assay for Lipases and Esterases

Mixed carbonates of 7‐hydroxy‐4‐methylcoumarin were shown to be a new class of probe for fluorogenic assays. They are promising substrates for fingerprinting enzyme hydrolytic activity, and proved particularly useful because of the low level of nonspecific degradation and ease of synthesis. They are highly relevant for screening lipase and esterase libraries. These advantages make umbelliferyl carbonates highly suitable substrates for high‐throughput screening. Moreover, we report the use of chiral fluorogenic carbonates as enantiopreference probes. Fluorogenic probes: Mixed carbonates of 7‐hydroxy‐4‐methylcoumarin constitute a new class of probe for fluorogenic assays. They represent promising substrates for fingerprinting enzyme hydrolytic activity. They proved particularly useful because of the low nonspecific degradation and their easy synthesis, and they proved highly relevant for screening lipase and esterase libraries.