The production of high-content fructo-oligosaccharides from sucrose by the mixed-enzyme system of fructosyltransferase and glucose oxidase

A technique to produce high-content fructo-oligosaccharides by the mixed-enzyme system of fructosyltransferase and glucose oxidase was investigated. The mixed-enzyme reaction was carried out in a stirred tank reactor containing 40% (w/v) sucrose with 10 unit of fructosyltransferase and 10 unit of glucose oxidase per gram sucrose for 25 h, at 40 degree C and pH 5.5. Highly concentrated fructo-oligosaccharides up to 90% was obtained by the mixed-enzyme system.