Transcription of the chicken anemia virus (CAV) genome and synthesis of its 52-kDa protein

This paper describes the expression of the chicken anemia virus (CAV) genome, a recently characterized single-stranded circular-DNA virus of a new type [Noteborn et al. J. Virol. 65 (1991) 3131-3139]. The major transcript from the CAV genome is an unspliced mRNA of about 2100 nucleotides (nt). Its t...

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Veröffentlicht in:	Gene 1992-09, Vol.118 (2), p.267-271
Hauptverfasser:	Noteborn, Mathieu H.M., Kranenburg, Onno, Zantema, Alt, Koch, Guus, de Boer, Gerben F., van der Eb, Alex J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Blotting, Northern chicken anemia virus DNA Viruses - genetics DNA, Viral - genetics dot-blot analysis Fundamental and applied biological sciences. Psychology Genes, Overlapping - genetics Genetics Genome, Viral in vitro translation Microbiology Northern blot overlapping ORFs polycistronic Restriction Mapping RNA transcript RNA, Messenger - genetics RNA, Viral - genetics S1 mapping Transcription, Genetic - genetics Viral Proteins - biosynthesis Viral Proteins - genetics Virology
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Zusammenfassung:	This paper describes the expression of the chicken anemia virus (CAV) genome, a recently characterized single-stranded circular-DNA virus of a new type [Noteborn et al. J. Virol. 65 (1991) 3131-3139]. The major transcript from the CAV genome is an unspliced mRNA of about 2100 nucleotides (nt). Its transcription start point and poly(A)-addition site are located at nt 354 and 2317 of the CAV sequence, respectively. In vitro translation experiments provide evidence that the major CAV open reading frame encodes a 52-kDa protein by using the fifth AUG as a start codon of the unspliced CAV mRNA.
ISSN:	0378-1119 1879-0038
DOI:	10.1016/0378-1119(92)90198-X