Nesterenkonia alkaliphila sp. nov., an alkaliphilic, halotolerant actinobacteria isolated from the western Pacific Ocean

A Gram-staining-positive, aerobic, motile and non-spore-forming actinobacteria, designated strain F10 ᵀ, was isolated from a deep-sea sediment of the western Pacific Ocean. Phylogenetic and phenotypic properties of the organism supported that it belonged to the genus Nesterenkonia . Strain F10 ᵀ shared highest 16S rRNA gene sequence similarity of 96.8 % with Nesterenkonia aethiopica DSM 17733 ᵀ, followed by Nesterenkonia xinjiangensis YIM 70097 ᵀ (96.7 %) and Nesterenkonia alba CAAS 252 ᵀ (96.6 %). The organism grew at 4–50 °C, at pH 7.0–12.0 and in the presence of 0–12 % (w/v) NaCl, with optimal growth occurring at 40 °C, at pH 9.0 and in the presence of 1 % (w/v) NaCl. The peptidoglycan type was A4(alpha), l -Lys–Gly– l -Glu. The polar lipid profile of strain F10 ᵀ consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unknown glycolipids and two unknown lipids. The isolate contained MK-9 (92 %) and MK-8 (5.8 %) as the major components of the menaquinone system, and anteiso-C ₁₇ : ₀ (50.9 %) and anteiso-C ₁₅ : ₀ (29.8 %) as the predominant fatty acids. The G+C content of the genomic DNA of strain F10 ᵀ was 66.2 mol%. Based on phenotypic, genotypic and phylogenetic analyses, strain F10 ᵀ represents a novel species of the genus Nesterenkonia for which the name Nesterenkonia alkaliphila sp. nov. is proposed. The type strain is F10 ᵀ (= LMG 28112 ᵀ = CGMCC 1.12781 ᵀ = JCM 19766 ᵀ = MCCC 1A09946 ᵀ).