Regulation of major histocompatibility complex class I expression by NF-κB-related proteins in breast cancer cells

Downregulation of MHC Class I antigens has been observed in many cancers and usually results from a decreased gene transcription. A reporter CAT gene dependent on the MHC Class I κB site or on a longer promoter is transactivated by NF-κB complexes containing p65 or RelB. p100 as well as IκB-α are po...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Oncogene 1998-06, Vol.16 (25), p.3299-3307
Hauptverfasser: DEJARDIN, E, DEREGOWSKI, V, GREIMERS, R, ZHENZI CAI, CHOUAIB, S, MERVILLE, M.-P, BOURS, V
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Downregulation of MHC Class I antigens has been observed in many cancers and usually results from a decreased gene transcription. A reporter CAT gene dependent on the MHC Class I κB site or on a longer promoter is transactivated by NF-κB complexes containing p65 or RelB. p100 as well as IκB-α are potent inhibitors of this transcription and p100 sequesters RelB and p65 complexes in the cytoplasm of breast cancer cells. However, although p100 is highly expressed in a number of breast cancer cell lines, MHC Class I antigen expression was observed on all the cell lines we analysed and could be further induced by stimulation with the cytokines IFN-γ or TNF-α. Stable transfection of a unresponsive mutated IκB-α Ser 32-36 expression vector showed that TNF-α induced MHC Cl I expression in an NF-κB-dependent way while IFN-γ did it independently of any NF-κB activation.
ISSN:0950-9232
1476-5594
DOI:10.1038/sj.onc.1201879