Absolute qPCR for Measuring Telomere Length in Bone Marrow Samples of Plasma Cell Disorders

Telomere length (TL) is currently used as an emerging biomarker in understanding the development/progression of hematological malignancies. The absolute quantitative PCR (qPCR) methodology has allowed the study of TL from a variety of mammalian tissues, but it has not been tested for bone marrow (BM...

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Veröffentlicht in:	Molecular biotechnology 2015-02, Vol.57 (2), p.155-159
Hauptverfasser:	Panero, Julieta, O’Callaghan, Nathan J, Fenech, Michael, Slavutsky, Irma
Format:	Artikel
Sprache:	eng
Schlagworte:	Biochemistry Biological Techniques biomarkers Biotechnology Bone marrow Bone Marrow Cells - pathology Cell Biology Cellular biology Chemistry Chemistry and Materials Science Disease Progression DNA Hematologic Neoplasms - diagnosis Hematologic Neoplasms - genetics Hematologic Neoplasms - pathology Human Genetics Humans Multiple myeloma pathogenesis patients Plasma Plasma Cells Polymerase Chain Reaction prognosis Protein Science quantitative polymerase chain reaction Telomere - genetics Telomere Homeostasis - genetics telomeres
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Beschreibung
Zusammenfassung:	Telomere length (TL) is currently used as an emerging biomarker in understanding the development/progression of hematological malignancies. The absolute quantitative PCR (qPCR) methodology has allowed the study of TL from a variety of mammalian tissues, but it has not been tested for bone marrow (BM) samples. In this study, we have examined the relationship between TL data generated by absolute qPCR versus those obtained by terminal restriction fragments (TRF) in 102 BM samples from patients with plasma cell disorders. A significant linear correlation between both methodologies was observed (p
ISSN:	1073-6085 1559-0305
DOI:	10.1007/s12033-014-9811-8