Effect of cooling on sperm motility before and after frozen-thawed stallion semen

Effect of cooling on sperm motility before and after frozen-thawed stallion semen

The aim of this study was to assess the effect of cooling on sperm motility before and after frozen-thawed stallion semen. Fifteen ejaculates of three stallions were collected with artificial vagina. The progressive motility was determined under microscope immediately after collection, cooling (5[de...

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Veröffentlicht in:African journal of biotechnology 2014-03, Vol.13 (12), p.1439-1443
Hauptverfasser: R., A. Ledezma-Torres, L., Sandoval, P., Valladares, M., Mellado, F., J. Picón, R., Ramírez, F., Sánchez
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container_end_page 1443
container_issue 12
container_start_page 1439
container_title African journal of biotechnology
container_volume 13
creator R., A. Ledezma-Torres
L., Sandoval
P., Valladares
M., Mellado
F., J. Picón
R., Ramírez
F., Sánchez
description The aim of this study was to assess the effect of cooling on sperm motility before and after frozen-thawed stallion semen. Fifteen ejaculates of three stallions were collected with artificial vagina. The progressive motility was determined under microscope immediately after collection, cooling (5[degrees]C for 0, 2, 7 or 24 h) before frozen-thawed and cooling (5[degrees]C for 0, 2, 7 or 24 h) after the semen was frozen-thawed. Sperm progressive motility (83.1, 78.7, 74.8 or 70.3%, respectively) was significantly different (P
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Similar pattern was found when semen was subjected to cooling, frozen-thawed and cooling time resulted in a progressive reduction in motility from 39.4 to 26.9%. The motility of semen subjected only to cooling for 24 h before freezing was optimal (70.0%) for artificial insemination. 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title Effect of cooling on sperm motility before and after frozen-thawed stallion semen
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